Affinity Chromatography: Methods and Protocols (Online; Springer) by
Affinity chromatography is a powerful tool, with exquisite specificity, for the purification of biomolecules. This comprehensive collection of detailed affinity chromatography methods provides all the information necessary to use these powerful techniques successfully in the laboratory and in real world situations. The collection includes classic, current, and state-of-the-art methods, all written by experts who have perfected them in their laboratories. The techniques range from small molecule to protein ligands, and to supra-molecular structures in phage display.
Affinity Chromatography: Methods and protocols (Online; Springer) by
The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols, molecular modelling, and the study of ligand-target interactions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Affinity Chromatography: Methods and Protocols, Third Edition is designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity.
Affinity Chromatography: Methods and Protocols (Online; Springer) by
Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. It contains state-of-the-art, brand new protocols, and provides step-by-step laboratory instructions for readily reproducible results.
Watch the JoVE Video: Chromatography-based Biomolecule Purification Methods
